Hotspot

trnH-GUG-psbA

LSC · 148-414 · 267 bp

Back to Phyllanthus

Pi 0.1

MarkerSeek score 76.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 64
pi: 0.1
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	714-733 bp	0.666667	75.0
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	713-732 bp	0.666667	75.0
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	728-747 bp	0.666667	74.8
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	727-746 bp	0.666667	74.8
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	729-748 bp	0.666667	74.8