Hotspot

ndhE-ndhG

SSC · 119410-119631 · 222 bp

Back to Phyllagathis

Pi 0.0282

MarkerSeek score 79.80

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 222
variable_sites: 52
pi: 0.0282
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGACTCGTGATCAATCCA	CAATTGTTGTTGCCCGTCAA	45.0	45.0	56.7	57.8	266-332 bp	1.0	79.7
ndhE-ndhG_p2	TTCGACTCGTGATCAATCCA	ATTGTTGTTGCCCGTCAATG	45.0	45.0	56.7	57.6	264-330 bp	1.0	78.0
ndhE-ndhG_p3	TCGACTCGTGATCAATCCAT	CAATTGTTGTTGCCCGTCAA	45.0	45.0	56.5	57.8	265-331 bp	1.0	77.9
ndhE-ndhG_p4	TTTCGACTCGTGATCAATCCA	CAATTGTTGTTGCCCGTCAA	42.9	45.0	57.4	57.8	267-333 bp	1.0	77.6
ndhE-ndhG_p5	TCCAAACACATAAGAGCCCT	CAATTGTTGTTGCCCGTCAA	45.0	45.0	56.4	57.8	294-360 bp	0.909091	74.1