Hotspot

ccsA-ndhD

SSC · 116651-116969 · 319 bp

Back to Phyllagathis

Pi 0.036

MarkerSeek score 78.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 319
variable_sites: 96
pi: 0.036
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCTATGGGCTTTC	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.9	57.6	463-518 bp	1.0	87.4
ccsA-ndhD_p2	TGTGGCTTCTATGGGCTTTC	CAGTTGAGAAGGTCGAAGCT	50.0	50.0	57.9	57.6	462-517 bp	1.0	87.4
ccsA-ndhD_p3	GATTGTGGCTTCTATGGGCT	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.6	57.6	466-521 bp	1.0	85.6
ccsA-ndhD_p4	GATTGTGGCTTCTATGGGCT	CAGTTGAGAAGGTCGAAGCT	50.0	50.0	57.6	57.6	465-520 bp	1.0	85.6
ccsA-ndhD_p5	GTGGCTTCTATGGGCTTTCT	TCAGTTGAGAAGGTCGAAGC	50.0	50.0	57.6	57.6	462-517 bp	1.0	85.1