Hotspot

ndhF-rpl32

SSC · 114047-115060 · 1014 bp

Back to Phyllagathis

Pi 0.0451

MarkerSeek score 85.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1014
variable_sites: 315
pi: 0.0451
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCATTGGAAAAACGA	TGAAAAAGCCTTCAATGCTGT	40.0	38.1	56.7	56.9	1024-1777 bp	0.909091	61.5
ndhF-rpl32_p2	AGGAATGGGAAGTGGAATGAG	TGAAAAAGCCTTCAATGCTGT	47.6	38.1	57.3	56.9	982-1735 bp	0.954545	60.1
ndhF-rpl32_p3	TTGTTGCCATTGGAAAAACGA	TGAAAAAGCCTTCAATGCTGT	38.1	38.1	57.5	56.9	1025-1778 bp	0.909091	59.3
ndhF-rpl32_p4	GGAATGGGAAGTGGAATGAGA	TGAAAAAGCCTTCAATGCTGT	47.6	38.1	57.3	56.9	981-1734 bp	0.931818	59.2
ndhF-rpl32_p5	TGTTGCCATTGGAAAAACGAT	TGAAAAAGCCTTCAATGCTGT	38.1	38.1	57.3	56.9	1024-1777 bp	0.909091	57.6