Hotspot

rpl14-rps3

LSC · 82195-83890 · 1696 bp

Back to Phyllagathis

Pi 0.0125

MarkerSeek score 78.40

Primer available yes

Label rpl14-rps3

Feature Metadata

label_name: rpl14-rps3
feature_type: igs
region: LSC
length_bp: 1696
variable_sites: 235
pi: 0.0125
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps3_p1	CGCGACATTCAAATGGGTTT	CAGTCCGAACGATCTATGGG	45.0	55.0	57.7	57.6	1781-1930 bp	1.0	85.8
rpl14-rps3_p2	CGCGACATTCAAATGGGTTT	CTCTACAAACCATTCGGGCT	45.0	50.0	57.7	57.6	1823-1972 bp	0.977273	85.1
rpl14-rps3_p3	CACATCAATTTTCGAGCCCC	CAGTCCGAACGATCTATGGG	50.0	55.0	57.4	57.6	1810-1959 bp	1.0	84.1
rpl14-rps3_p4	CGCGACATTCAAATGGGTTT	CCTCTACAAACCATTCGGGC	45.0	55.0	57.7	58.6	1824-1973 bp	0.977273	83.4
rpl14-rps3_p5	CACATCAATTTTCGAGCCCC	CTCTACAAACCATTCGGGCT	50.0	50.0	57.4	57.6	1852-2001 bp	0.977273	83.3