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rps8-rpl14

LSC · 81518-81825 · 308 bp

Back to Phyllagathis

Pi 0.0305

MarkerSeek score 78.60

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 97
pi: 0.0305
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACGTCAGCAATAGTGTCTCT	GCCCGAGAATTGAGACAGTT	45.0	50.0	56.3	57.9	339-455 bp	1.0	77.6
rps8-rpl14_p2	ACGTCAGCAATAGTGTCTCT	CGATTGCCCGAGAATTGAGA	45.0	50.0	56.3	58.1	344-460 bp	0.977273	77.0
rps8-rpl14_p3	ACGTCAGCAATAGTGTCTCT	TTGCCCGAGAATTGAGACAG	45.0	50.0	56.3	57.9	341-457 bp	0.977273	76.7
rps8-rpl14_p4	GTCAGCAATAGTGTCTCTACCC	CGATTGCCCGAGAATTGAGA	50.0	50.0	58.0	58.1	342-458 bp	0.977273	74.2
rps8-rpl14_p5	GTCAGCAATAGTGTCTCTACCC	TTGCCCGAGAATTGAGACAG	50.0	50.0	58.0	57.9	339-455 bp	0.977273	73.9