Hotspot

petA-psbJ

LSC · 63510-64414 · 905 bp

Back to Phyllagathis

Pi 0.0216

MarkerSeek score 86.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 197
pi: 0.0216
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.6	58.1	612-1086 bp	0.977273	87.0
petA-psbJ_p2	TCCATTACGTGTCCAAGGTC	ATACTCAGGGTTGGGCTCAT	50.0	50.0	57.2	58.1	618-1092 bp	1.0	85.2
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.6	58.2	954-1090 bp	0.954545	84.9
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	TTCATACTCAGGGTTGGGCT	45.0	50.0	57.6	58.3	953-1089 bp	0.954545	84.2
petA-psbJ_p5	ACGTGTCCAAGGTCTTTTGT	GGTTCATACTCAGGGTTGGG	45.0	55.0	57.6	57.6	955-1091 bp	0.954545	83.5