Hotspot

accD-psaI

LSC · 59326-60172 · 847 bp

Back to Phyllagathis

Pi 0.0194

MarkerSeek score 84.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 161
pi: 0.0194
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	756-1024 bp	1.0	78.2
accD-psaI_p2	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	906-1025 bp	0.977273	77.3
accD-psaI_p3	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	757-1025 bp	1.0	76.3
accD-psaI_p4	AAGGGTGTTCTGAGTGAGTT	AAAGACGCCATTGCAATTGC	45.0	45.0	56.3	58.6	763-1031 bp	1.0	73.8
accD-psaI_p5	AAAGGGTGTTCTGAGTGAGT	AAAGACGCCATTGCAATTGC	45.0	45.0	56.3	58.6	913-1032 bp	0.977273	72.9