Hotspot

trnD-GUC-trnY-GUA

LSC · 30774-31264 · 491 bp

Back to Phyllagathis

Pi 0.027
MarkerSeek score 72.90
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
491
variable_sites
107
pi
0.027
unique_haplotype_count
44
primer_available
yes
alignment_reliability
0.93
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 122-596 bp 0.909091 82.0
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 121-595 bp 0.909091 82.0
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.6 57.5 138-612 bp 0.909091 79.8
trnD-GUC-trnY-GUA_p4 TTATTCCTCGGGACTGACGG TGGCAATATGTCTACGCTGG 55.0 50.0 58.9 57.8 173-625 bp 0.295455 50.2
trnD-GUC-trnY-GUA_p5 TTATTCCTCGGGACTGACGG GGCAATATGTCTACGCTGGT 55.0 50.0 58.9 57.8 172-624 bp 0.295455 50.2