Hotspot

atpF-atpH

LSC · 13073-13583 · 511 bp

Back to Phyllagathis

Pi 0.018

MarkerSeek score 82.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 98
pi: 0.018
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GGATTGGTTGTCGCTTTAGC	50.0	50.0	57.9	57.5	617-655 bp	1.0	86.7
atpF-atpH_p2	GGCCCAAGGAAAGGAAAGAA	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.0	57.5	585-623 bp	0.931818	84.8
atpF-atpH_p3	GGCCCAAGGAAAGGAAAGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	650-688 bp	0.931818	83.7
atpF-atpH_p4	GGCCCAAGGAAAGGAAAGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	651-689 bp	0.909091	82.7
atpF-atpH_p5	GGCCCAAGGAAAGGAAAGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	652-690 bp	0.909091	82.7