Hotspot

rps16-trnQ-UUG

LSC · 5723-6732 · 1010 bp

Back to Phyllagathis

Pi 0.0225
MarkerSeek score 78.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1010
variable_sites
258
pi
0.0225
unique_haplotype_count
44
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 828-1093 bp 1.0 85.1
rps16-trnQ-UUG_p2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 831-1096 bp 1.0 82.9
rps16-trnQ-UUG_p3 TAAGAATCCACGGACGATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 56.8 58.1 864-1129 bp 1.0 82.0
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.2 57.3 832-1097 bp 1.0 80.4
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG TAAGGCAACGGGTTTTGGTC 50.0 50.0 57.2 58.7 858-1123 bp 0.977273 79.4