Hotspot

trnH-GUG-psbA

LSC · 76-311 · 236 bp

Back to Phyllagathis

Pi 0.0637

MarkerSeek score 90.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 236
variable_sites: 83
pi: 0.0637
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 90.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	268-366 bp	0.931818	88.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	282-380 bp	0.931818	87.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	281-379 bp	0.931818	87.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	283-381 bp	0.931818	87.9
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	218-316 bp	0.886364	85.9