Hotspot

rps15-ycf1

SSC · 134528-135148 · 621 bp

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Pi 0.00886

MarkerSeek score 63.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 621
variable_sites: 13
pi: 0.00886
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGAACCCCTGTTTTCTTCT	GCTTGCATGAATCGCTATTGG	45.0	47.6	55.2	58.3	567-802 bp	1.0	52.0
rps15-ycf1_p2	ACTTGAAATTCAACAGAACCCC	GCTTGCATGAATCGCTATTGG	40.9	47.6	57.1	58.3	580-815 bp	1.0	51.1
rps15-ycf1_p3	CAGAACCCCTGTTTTCTTCTT	GCTTGCATGAATCGCTATTGG	42.9	47.6	56.0	58.3	567-802 bp	1.0	50.5
rps15-ycf1_p4	CAGAACCCCTGTTTTCTTCT	AGCTTGCATGAATCGCTATTG	45.0	42.9	55.2	57.4	568-803 bp	1.0	49.8
rps15-ycf1_p5	CAGAACCCCTGTTTTCTTCT	TGGCCCAATTACCGATTAGA	45.0	45.0	55.2	56.3	594-829 bp	1.0	49.1