Hotspot

ycf1-ndhF

SSC · 118912-119013 · 102 bp

Back to Phrynium

Pi 0.0624

MarkerSeek score 77.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 14
pi: 0.0624
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AACGGAAGAAAGGGGAGAGA	GGGTTGGTGTTGCAAGTTTC	50.0	50.0	58.0	58.1	578-585 bp	0.4	58.4
ycf1-ndhF_p2	AGAATGCCGTCGCCTATTTT	GGGTTGGTGTTGCAAGTTTC	45.0	50.0	58.0	58.1	625-632 bp	0.4	58.1
ycf1-ndhF_p3	GCCGTCGCCTATTTTCACTA	GGGTTGGTGTTGCAAGTTTC	50.0	50.0	58.1	58.1	620-627 bp	0.4	57.9
ycf1-ndhF_p4	CACCGAAGAAGACCTTTCCC	GGGTTGGTGTTGCAAGTTTC	55.0	50.0	58.2	58.1	491-498 bp	0.4	57.7
ycf1-ndhF_p5	CCACCGAAGAAGACCTTTCC	GGGTTGGTGTTGCAAGTTTC	55.0	50.0	58.2	58.1	492-499 bp	0.4	57.7