Hotspot

rpl22-rps19

IRb · 91802-92072 · 271 bp

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Pi 0.0284

MarkerSeek score 64.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 271
variable_sites: 17
pi: 0.0284
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACTTCTTTACCCGAACTCAA	TGCAAGAAACGATAATAAATCTCGT	40.0	32.0	54.2	57.5	255-338 bp	1.0	48.3
rpl22-rps19_p2	ACTTCTTTACCCGAACTCAA	AGAAACGATAATAAATCTCGTCGT	40.0	33.3	54.2	56.4	251-334 bp	1.0	48.3
rpl22-rps19_p3	ACTTCTTTACCCGAACTCAAA	TGCAAGAAACGATAATAAATCTCGT	38.1	32.0	55.1	57.5	255-338 bp	1.0	48.3
rpl22-rps19_p4	ACTTCTTTACCCGAACTCAAA	AGAAACGATAATAAATCTCGTCGT	38.1	33.3	55.1	56.4	251-334 bp	1.0	48.3
rpl22-rps19_p5	ACTTCTTTACCCGAACTCAA	GAAACGATAATAAATCTCGTCGT	40.0	34.8	54.2	55.0	250-333 bp	1.0	48.3