Hotspot

petA-psbJ

LSC · 70371-71376 · 1006 bp

Back to Phrynium

Pi 0.00934

MarkerSeek score 62.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1006
variable_sites: 23
pi: 0.00934
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GATCCATTACGGGTCCAAGG	TCTGGGTTGGGTTCATCTCT	55.0	50.0	57.7	58.0	1149-1150 bp	1.0	79.3
petA-psbJ_p2	GATCCATTACGGGTCCAAGG	TGGGTTGGGTTCATCTCTCT	55.0	50.0	57.7	58.0	1147-1148 bp	1.0	79.3
petA-psbJ_p3	CGGGTCCAAGGTCTTTTGTT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.3	58.0	1140-1141 bp	1.0	78.8
petA-psbJ_p4	CGGGTCCAAGGTCTTTTGTT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.3	58.0	1138-1139 bp	1.0	78.8
petA-psbJ_p5	ATCCATTACGGGTCCAAGGT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.4	58.0	1146-1147 bp	1.0	78.1