Hotspot

trnE-UUC-trnT-GGU

LSC · 36081-37106 · 1026 bp

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Pi 0.0105

MarkerSeek score 71.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1026
variable_sites: 23
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	947-1135 bp	1.0	84.5
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	945-1133 bp	1.0	84.5
trnE-UUC-trnT-GGU_p3	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	999-1187 bp	1.0	84.4
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	924-1112 bp	1.0	84.1
trnE-UUC-trnT-GGU_p5	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	978-1166 bp	1.0	84.0