Hotspot

atpH-atpI

LSC · 16732-18395 · 1664 bp

Back to Phrynium

Pi 0.0133

MarkerSeek score 63.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1664
variable_sites: 50
pi: 0.0133
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1614-1747 bp	1.0	85.8
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	57.9	58.0	1630-1763 bp	1.0	84.9
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	1615-1748 bp	1.0	84.7
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.8	58.0	1629-1762 bp	1.0	84.4
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.3	58.0	1624-1757 bp	1.0	84.0