Hotspot

rps16-trnQ-UUG

LSC · 6619-7789 · 1171 bp

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Pi 0.00669

MarkerSeek score 61.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1171
variable_sites: 15
pi: 0.00669
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	755-1262 bp	1.0	84.2
rps16-trnQ-UUG_p2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	753-1260 bp	1.0	84.2
rps16-trnQ-UUG_p3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	761-1268 bp	1.0	84.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	737-1244 bp	1.0	83.3
rps16-trnQ-UUG_p5	CAGCCGATCATGTCCTTCAA	CCAAACAGAAAAATGGGGCG	50.0	50.0	58.0	58.2	816-1323 bp	1.0	83.1