Hotspot

rpl2

IRa · 151779-153270 · 1492 bp

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Pi 0
MarkerSeek score 25.80
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
IRa
length_bp
1492
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 GAATGGTTAAAGCGCCCAAC TGTAAAGACGAAGAAAGAAATTGA 50.0 29.2 58.0 55.0 2053 bp 0.5 20.0
rpl2_p2 ACTTCCGGGAAAGGGTAGAA TGTAAAGACGAAGAAAGAAATTGA 50.0 29.2 58.0 55.0 1665 bp 0.5 20.0
rpl2_p3 AGCATTGGGTCGAACTCTTC TGTAAAGACGAAGAAAGAAATTGA 50.0 29.2 57.9 55.0 1720 bp 0.5 20.0
rpl2_p4 GCATTGGGTCGAACTCTTCT TGTAAAGACGAAGAAAGAAATTGA 50.0 29.2 57.9 55.0 1719 bp 0.5 20.0
rpl2_p5 TAGTCATCGACTTCCGGGAA TGTAAAGACGAAGAAAGAAATTGA 50.0 29.2 57.9 55.0 1674 bp 0.5 20.0