Hotspot

rpl16

LSC · 82108-83386 · 1279 bp

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Pi 0.000786
MarkerSeek score 59.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1279
variable_sites
1
pi
0.000786
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TGTTTCATCCCCAAGACTCA ACTCGTTCATTCTTTTTATAGCCA 45.0 33.3 56.4 56.7 1402-1417 bp 1.0 40.9
rpl16_p2 TTGTTTCATCCCCAAGACTCA ACTCGTTCATTCTTTTTATAGCCA 42.9 33.3 57.1 56.7 1403-1418 bp 1.0 40.9
rpl16_p3 TGTTTCATCCCCAAGACTCA AACTCGTTCATTCTTTTTATAGCCA 45.0 32.0 56.4 57.3 1403-1418 bp 1.0 40.9
rpl16_p4 TGTTTCATCCCCAAGACTCA ACTCGTTCATTCTTTTTATAGCCAA 45.0 32.0 56.4 57.3 1402-1417 bp 1.0 40.9
rpl16_p5 TGTTTCATCCCCAAGACTCAT ACTCGTTCATTCTTTTTATAGCCA 42.9 33.3 56.9 56.7 1402-1417 bp 1.0 40.9