Hotspot

ndhF

SSC · 110356-112599 · 2244 bp

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Pi 0.00134
MarkerSeek score 48.20
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2244
variable_sites
3
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TCTATGGCTCGGATCTTTAGT AAAGAGGTAAGGTCCGGTGA 42.9 50.0 55.7 58.0 2371 bp 1.0 53.3
ndhF_p2 TCTATGGCTCGGATCTTTAGT AAGAGGTAAGGTCCGGTGAA 42.9 50.0 55.7 58.0 2370 bp 1.0 53.3
ndhF_p3 TCTATGGCTCGGATCTTTAGT AGGTAAGGTCCGGTGAATCA 42.9 50.0 55.7 58.1 2367 bp 1.0 53.1
ndhF_p4 TGGCTCGGATCTTTAGTATTCTC AAAGAGGTAAGGTCCGGTGA 43.5 50.0 57.5 58.0 2367 bp 1.0 52.3
ndhF_p5 TGGCTCGGATCTTTAGTATTCTC AAGAGGTAAGGTCCGGTGAA 43.5 50.0 57.5 58.0 2366 bp 1.0 52.3