Hotspot

ndhA

SSC · 119431-121602 · 2172 bp

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Pi 0.00138
MarkerSeek score 52.10
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2172
variable_sites
3
pi
0.00138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGACCAGAATTGAAGACCTGAG GCCGGGTTTTATCAACTTGC 45.5 50.0 57.7 58.0 2400 bp 1.0 61.8
ndhA_p2 GACCAGAATTGAAGACCTGAGT GCCGGGTTTTATCAACTTGC 45.5 50.0 57.7 58.0 2399 bp 1.0 61.8
ndhA_p3 TGACCAGAATTGAAGACCTGAG AACTTGCAGATTCTTCCGCA 45.5 45.0 57.7 58.1 2387 bp 1.0 61.1
ndhA_p4 TGACCAGAATTGAAGACCTGAG TTGCAGATTCTTCCGCAGTT 45.5 45.0 57.7 58.1 2384 bp 1.0 61.1
ndhA_p5 GACCAGAATTGAAGACCTGAGT TTGCAGATTCTTCCGCAGTT 45.5 45.0 57.7 58.1 2383 bp 1.0 61.0