Hotspot

clpP1

LSC · 71011-72976 · 1966 bp

Pi 0.00407

MarkerSeek score 60.20

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 1966
variable_sites: 8
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGA	47.6	40.0	57.7	55.5	2133-2138 bp	1.0	50.1
clpP1_p2	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAAG	47.6	40.9	57.7	57.4	2133-2138 bp	1.0	49.6
clpP1_p3	TGGGCTGGTTTAGATTGATCC	CGTTTCCACATCAAAGTGAAGT	47.6	40.9	57.7	57.4	2132-2137 bp	1.0	49.6
clpP1_p4	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAA	47.6	38.1	57.7	56.3	2133-2138 bp	1.0	48.5
clpP1_p5	GGGCTGGTTTAGATTGATCCT	ACGTTTCCACATCAAAGTGA	47.6	40.0	57.4	55.5	2132-2137 bp	1.0	47.8