Hotspot

cemA

LSC · 62273-62962 · 690 bp

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Pi 0.00145
MarkerSeek score 59.60
Primer available yes
Label cemA

Feature Metadata

label_name
cemA
feature_type
gene
region
LSC
length_bp
690
variable_sites
1
pi
0.00145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA_p1 ACCTTGTCTCGAACTCATGT AGGATGAAAATCCCCTGGAT 45.0 45.0 56.5 55.8 885 bp 1.0 50.7
cemA_p2 ACCTTGTCTCGAACTCATGT CGATAGGATGAAAATCCCCTGG 45.0 50.0 56.5 58.3 889 bp 1.0 49.5
cemA_p3 ACCTTGTCTCGAACTCATGT ACGATAGGATGAAAATCCCCTG 45.0 45.5 56.5 57.6 890 bp 1.0 49.2
cemA_p4 CGACAAACGAAGTGGGTTAA AGGATGAAAATCCCCTGGAT 45.0 45.0 56.3 55.8 837 bp 1.0 49.2
cemA_p5 ACCTTGTCTCGAACTCATGTT AGGATGAAAATCCCCTGGAT 42.9 45.0 57.2 55.8 885 bp 1.0 48.7