Hotspot

trnS-GGA-rps4

LSC · 45987-46311 · 325 bp

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Pi 0.01
MarkerSeek score 62.70
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
325
variable_sites
3
pi
0.01
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 AGGCTTTTGTTTACCGAGGG TTTGACCCTTCACCCATTCC 50.0 50.0 58.1 57.7 457-483 bp 1.0 81.3
trnS-GGA-rps4_p2 AGGCTTTTGTTTACCGAGGG GACCCTTCACCCATTCCAAT 50.0 50.0 58.1 57.5 454-480 bp 1.0 79.5
trnS-GGA-rps4_p3 ATGTAGGAGAGATGGCCGAG TTTGACCCTTCACCCATTCC 55.0 50.0 58.4 57.7 509-535 bp 1.0 79.0
trnS-GGA-rps4_p4 GGCTTTTGTTTACCGAGGGT TTTGACCCTTCACCCATTCC 50.0 50.0 58.4 57.7 456-482 bp 1.0 79.0
trnS-GGA-rps4_p5 ATGTAGGAGAGATGGCCGAG GACCCTTCACCCATTCCAAT 55.0 50.0 58.4 57.5 506-532 bp 1.0 77.2