Hotspot

trnS-GCU-trnG-UCC

LSC · 9058-9765 · 708 bp

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Pi 0.00282
MarkerSeek score 60.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
708
variable_sites
2
pi
0.00282
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 814 bp 1.0 52.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCTTTAACT 50.0 40.0 58.0 54.5 813 bp 1.0 52.0
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 777 bp 1.0 51.9
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 776 bp 1.0 51.9
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA AACCGAAAGACCCTTTAACT 50.0 40.0 58.2 54.5 868 bp 1.0 50.9