Hotspot

rps16-trnQ-UUG

LSC · 6570-7759 · 1190 bp

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Pi 0.00173
MarkerSeek score 62.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1190
variable_sites
2
pi
0.00173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCCACAAGGGATCATGTCCT GAAATTCAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1333-1343 bp 1.0 81.8
rps16-trnQ-UUG_p2 TCCACAAGGGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1275-1285 bp 1.0 81.3
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAAATTCAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1311-1321 bp 1.0 80.9
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1253-1263 bp 1.0 80.5
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAAATTCAAATGGGGCGTGG 50.0 50.0 58.2 58.0 1322-1332 bp 1.0 80.4