Hotspot

trnH-GUG-psbA

LSC · 94-631 · 538 bp

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Pi 0.00391
MarkerSeek score 56.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
538
variable_sites
2
pi
0.00391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 578-604 bp 1.0 82.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 592-618 bp 1.0 82.2
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 591-617 bp 1.0 82.2
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 593-619 bp 1.0 82.2
trnH-GUG-psbA_p5 TAGACAATCATGGGCGAACG TTCCCTCTAGACCTAGCTGC 50.0 55.0 58.1 57.9 669 bp 0.5 58.9