Hotspot

rpl16

LSC · 79471-80952 · 1482 bp

Back to Phragmites

Pi 0.00225

MarkerSeek score 60.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 5
pi: 0.00225
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCTGGGG	AAGAGCCTCATTTTTCCCGA	47.6	45.0	58.0	57.1	1625-1634 bp	1.0	65.2
rpl16_p2	TCCAGAAAGAAAAACCTGGGG	AGAGCCTCATTTTTCCCGAA	47.6	45.0	58.0	57.1	1624-1633 bp	1.0	65.2
rpl16_p3	TCCAGAAAGAAAAACCTGGGG	CAAGAGCCTCATTTTTCCCG	47.6	50.0	58.0	57.1	1626-1635 bp	1.0	64.8
rpl16_p4	TCCAGAAAGAAAAACCTGGGG	AAGAGCCTCATTTTTCCCGAA	47.6	42.9	58.0	57.8	1625-1634 bp	1.0	63.0
rpl16_p5	TCCAGAAAGAAAAACCTGGGG	AGAGCCTCATTTTTCCCGAAA	47.6	42.9	58.0	57.8	1624-1633 bp	1.0	63.0