Hotspot

petA

LSC · 61228-62190 · 963 bp

Back to Phragmites

Pi 0.000692

MarkerSeek score 42.00

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.000692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	TTGTAGAAATTCCGGGCTCC	TGATGGTAAGAAATCCCGGG	50.0	50.0	57.6	57.0	1015 bp	1.0	68.6
petA_p2	TTGTAGAAATTCCGGGCTCC	TTTTTGTTCTCGCCTCTGCC	50.0	50.0	57.6	59.0	1101 bp	1.0	68.4
petA_p3	TTGTAGAAATTCCGGGCTCC	AGGAATTCCAGAAATCGAGGC	50.0	47.6	57.6	58.1	1048 bp	1.0	68.2
petA_p4	TTGTAGAAATTCCGGGCTCC	GGAATTCCAGAAATCGAGGCT	50.0	47.6	57.6	58.1	1047 bp	1.0	68.2
petA_p5	TTGTAGAAATTCCGGGCTCC	CCTCTGCCTTGGATTCTCTTC	50.0	52.4	57.6	58.1	1089 bp	1.0	68.1