Hotspot

ndhK

LSC · 50950-51696 · 747 bp

Back to Phragmites

Pi 0.00268

MarkerSeek score 60.50

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 747
variable_sites: 3
pi: 0.00268
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TCTGCACAAAAGAACCCCTC	TCTCTACCCTTGGGCAATGA	50.0	50.0	58.0	58.0	887 bp	1.0	79.8
ndhK_p2	TCTGCACAAAAGAACCCCTC	CGACGTATTGGGTGTATCCG	50.0	55.0	58.0	58.2	863 bp	1.0	78.2
ndhK_p3	GATAACCAACCCTGCTGCAT	TCTCTACCCTTGGGCAATGA	50.0	50.0	58.2	58.0	985 bp	1.0	78.1
ndhK_p4	ATAACCAACCCTGCTGCATT	TCTCTACCCTTGGGCAATGA	45.0	50.0	57.8	58.0	984 bp	1.0	78.1
ndhK_p5	TCTGCACAAAAGAACCCCTC	GGGCAATGAGTTTCGACGTA	50.0	50.0	58.0	58.3	876 bp	1.0	77.9