Hotspot

ndhH

SSC · 115108-116289 · 1182 bp

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Pi 0.00113

MarkerSeek score 59.50

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCCAGTAGGAGGGTCAAAA	TGCATTTTGATGAGCCTCGT	50.0	45.0	57.6	58.2	1376-1379 bp	1.0	75.3
ndhH_p2	CAGTAGGAGGGTCAAAATGGG	TGCATTTTGATGAGCCTCGT	52.4	45.0	58.0	58.2	1373-1376 bp	1.0	70.7
ndhH_p3	CCAGTAGGAGGGTCAAAATGG	TGCATTTTGATGAGCCTCGT	52.4	45.0	58.0	58.2	1374-1377 bp	1.0	70.7
ndhH_p4	CCCAGTAGGAGGGTCAAAATG	TGCATTTTGATGAGCCTCGT	52.4	45.0	58.0	58.2	1375-1378 bp	1.0	70.7
ndhH_p5	AGGAGGGTCAAAATGGGTAGA	TGCATTTTGATGAGCCTCGT	47.6	45.0	58.1	58.2	1369-1372 bp	1.0	70.2