Hotspot

matK

LSC · 1673-3214 · 1542 bp

Back to Phragmites

Pi 0.0013

MarkerSeek score 59.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1542
variable_sites: 3
pi: 0.0013
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AGGGGAACATTTCAGAATGGA	CAACACCCTGTTCTGACCAT	42.9	50.0	56.9	57.7	1711 bp	1.0	61.0
matK_p2	AGGGGAACATTTCAGAATGGA	AATGCAACACCCTGTTCTGA	42.9	45.0	56.9	57.6	1715 bp	1.0	60.3
matK_p3	AGGGGAACATTTCAGAATGGA	ATGCAACACCCTGTTCTGAC	42.9	50.0	56.9	58.4	1714 bp	1.0	60.1
matK_p4	AGGGGAACATTTCAGAATGGAA	CAACACCCTGTTCTGACCAT	40.9	50.0	57.6	57.7	1711 bp	1.0	58.7
matK_p5	AGGGGAACATTTCAGAATGGAA	AATGCAACACCCTGTTCTGA	40.9	45.0	57.6	57.6	1715 bp	1.0	58.0