Hotspot

rpl32-trnL-UAG

SSC · 106884-107394 · 511 bp

Back to Phragmites

Pi 0.00913

MarkerSeek score 50.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 511
variable_sites: 7
pi: 0.00913
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAACGAGCATCCAAAACCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.2	57.9	602 bp	1.0	73.6
rpl32-trnL-UAG_p2	CAACGAGCATCCAAAACCAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.2	57.8	595 bp	1.0	73.1
rpl32-trnL-UAG_p3	CAACGAGCATCCAAAACCAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.2	58.2	616 bp	1.0	73.1
rpl32-trnL-UAG_p4	CAACGAGCATCCAAAACCAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.2	57.8	601 bp	1.0	73.0
rpl32-trnL-UAG_p5	CAACGAGCATCCAAAACCAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.2	57.8	600 bp	1.0	73.0