Hotspot

trnC-GCA-rpoB

LSC · 20239-21386 · 1148 bp

Back to Phragmites

Pi 0.00436

MarkerSeek score 62.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 7
pi: 0.00436
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTCTTCTGCCAAGCCTTGAT	50.0	50.0	58.1	57.9	1293-1296 bp	1.0	79.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1232-1235 bp	1.0	77.7
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1231-1234 bp	1.0	77.7
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1215-1218 bp	1.0	77.0
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.1	57.6	1216-1219 bp	1.0	76.8