Hotspot

trnD-GUC-psbM

LSC · 17206-18281 · 1076 bp

Back to Phragmites

Pi 0.00248

MarkerSeek score 63.40

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 4
pi: 0.00248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	1212-1241 bp	1.0	67.9
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.5	56.5	1214-1243 bp	1.0	66.1
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.5	56.5	1213-1242 bp	1.0	66.1
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.5	57.4	1212-1241 bp	1.0	65.9
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	TTTGGCTGGCTGTTTTTACA	50.0	40.0	58.5	56.3	1208-1237 bp	1.0	65.1