Hotspot

trnK-UUU-rps16

LSC · 3993-4425 · 433 bp

Back to Phragmites

Pi 0.0103
MarkerSeek score 53.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
433
variable_sites
5
pi
0.0103
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 ACCAAAATGAACAGATCCGGT GGTGCTCAACCTACAAGAACT 42.9 47.6 57.8 57.9 518-539 bp 1.0 65.7
trnK-UUU-rps16_p2 ACCAAAATGAACAGATCCGGT GGTGCTCAACCTACAAGAAC 42.9 50.0 57.8 56.4 518-539 bp 1.0 61.6
trnK-UUU-rps16_p3 ACCAAAATGAACAGATCCGGT TGCTCAACCTACAAGAACTGT 42.9 42.9 57.8 57.2 516-537 bp 1.0 60.0
trnK-UUU-rps16_p4 ACCAAAATGAACAGATCCGG GGTGCTCAACCTACAAGAACT 45.0 47.6 56.0 57.9 518-539 bp 1.0 59.1
trnK-UUU-rps16_p5 CCAAAATGAACAGATCCGGT GGTGCTCAACCTACAAGAACT 45.0 47.6 56.0 57.9 517-538 bp 1.0 59.1