Hotspot

ndhF-trnL-CAA

IRa · 135941-137051 · 1111 bp

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Pi 0.032
MarkerSeek score 54.50
Primer available yes
Label ndhF-trnL-CAA

Feature Metadata

label_name
ndhF-trnL-CAA
feature_type
igs
region
IRa
length_bp
1111
variable_sites
60
pi
0.032
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.63
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-CAA_p1 TTCATGGTTTATCTGGGGG GTCTCGCGTGTCTACCATTT 47.4 50.0 54.0 58.0 1166-1186 bp 0.4 16.3
ndhF-trnL-CAA_p2 TTCATGGTTTATCTGGGGG CTCGCGTGTCTACCATTTCA 47.4 50.0 54.0 58.0 1164-1184 bp 0.4 16.3
ndhF-trnL-CAA_p3 TTCATGGTTTATCTGGGGG GCGTGTCTACCATTTCACCA 47.4 50.0 54.0 58.2 1161-1181 bp 0.4 16.1
ndhF-trnL-CAA_p4 TTCATGGTTTATCTGGGGG TCTCGCGTGTCTACCATTTC 47.4 50.0 54.0 57.7 1165-1185 bp 0.4 16.1
ndhF-trnL-CAA_p5 TTCATGGTTTATCTGGGGG TCGCGTGTCTACCATTTCAC 47.4 50.0 54.0 58.3 1163-1183 bp 0.4 16.1