Hotspot

psaC-ndhI

SSC · 114622-115217 · 596 bp

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Pi 0.0439

MarkerSeek score 82.60

Primer available yes

Label psaC-ndhI

Feature Metadata

label_name: psaC-ndhI
feature_type: igs
region: SSC
length_bp: 596
variable_sites: 53
pi: 0.0439
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhI_p1	AATACATCCGTGGGACAAGC	TGGGTTCATGAATTATGGCA	50.0	40.0	58.0	55.0	728-1325 bp	1.0	62.6
psaC-ndhI_p2	GGGACAAGCTCGTACACATT	TGGGTTCATGAATTATGGCA	50.0	40.0	57.9	55.0	717-1314 bp	1.0	62.3
psaC-ndhI_p3	AATACATCCGTGGGACAAGC	GGGTTCATGAATTATGGCAAACA	50.0	39.1	58.0	57.9	727-1324 bp	1.0	62.2
psaC-ndhI_p4	AATACATCCGTGGGACAAGC	TGGGTTCATGAATTATGGCAAAC	50.0	39.1	58.0	57.9	728-1325 bp	1.0	62.2
psaC-ndhI_p5	GGGACAAGCTCGTACACATT	GGGTTCATGAATTATGGCAAACA	50.0	39.1	57.9	57.9	716-1313 bp	1.0	61.9