Hotspot

rpl32-trnL-UAG

SSC · 110797-111353 · 557 bp

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Pi 0.0793
MarkerSeek score 81.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
557
variable_sites
76
pi
0.0793
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCTTCCTTCGTAACTCCAGG ATCCGCATAAAAGAGCTGCA 55.0 45.0 58.0 58.0 1792-2285 bp 1.0 89.9
rpl32-trnL-UAG_p2 GCTTCCTTCGTAACTCCAGG TCCGCATAAAAGAGCTGCAT 55.0 45.0 58.0 58.0 1791-2284 bp 1.0 89.9
rpl32-trnL-UAG_p3 GAGCTACTGAGGAACAACGG ATCCGCATAAAAGAGCTGCA 55.0 45.0 58.0 58.0 1872-2365 bp 1.0 89.8
rpl32-trnL-UAG_p4 GAGCTACTGAGGAACAACGG TCCGCATAAAAGAGCTGCAT 55.0 45.0 58.0 58.0 1871-2364 bp 1.0 89.8
rpl32-trnL-UAG_p5 TCCACCGGACAGTCAAAAAG TCCGCATAAAAGAGCTGCAT 50.0 45.0 58.0 58.0 1256-1743 bp 1.0 89.5