Hotspot

trnN-GUU-rpl32

IRb · 110135-110622 · 488 bp

Back to Phragmipedium

Pi 0.0492
MarkerSeek score 74.90
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
IRb
length_bp
488
variable_sites
47
pi
0.0492
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AAGCGAGCCCTTTCATTCAA TTTGACATAGAAGTACGTTTTT 45.0 27.3 58.1 52.0 640-760 bp 1.0 49.5
trnN-GUU-rpl32_p2 AAAGCGAGCCCTTTCATTCA TTTGACATAGAAGTACGTTTTT 45.0 27.3 58.1 52.0 641-761 bp 1.0 49.4
trnN-GUU-rpl32_p3 AAAGCGAGCCCTTTCATTCA TTTTGACATAGAAGTACGTTTT 45.0 27.3 58.1 52.0 642-762 bp 1.0 49.4
trnN-GUU-rpl32_p4 AAGCGAGCCCTTTCATTCAA TTTTGACATAGAAGTACGTTTT 45.0 27.3 58.1 52.0 641-761 bp 1.0 49.4
trnN-GUU-rpl32_p5 AAGCGAGCCCTTTCATTCAA TTTTTGACATAGAAGTACGTTT 45.0 27.3 58.1 52.0 642-762 bp 1.0 49.4