Hotspot

trnL-CAA-ndhF

IRb · 96189-97299 · 1111 bp

Back to Phragmipedium

Pi 0.032

MarkerSeek score 54.50

Primer available yes

Label trnL-CAA-ndhF

Feature Metadata

label_name: trnL-CAA-ndhF
feature_type: igs
region: IRb
length_bp: 1111
variable_sites: 60
pi: 0.032
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhF_p1	GTCTCGCGTGTCTACCATTT	TTCATGGTTTATCTGGGGG	50.0	47.4	58.0	54.0	1166-1186 bp	0.4	16.3
trnL-CAA-ndhF_p2	CTCGCGTGTCTACCATTTCA	TTCATGGTTTATCTGGGGG	50.0	47.4	58.0	54.0	1164-1184 bp	0.4	16.3
trnL-CAA-ndhF_p3	GCGTGTCTACCATTTCACCA	TTCATGGTTTATCTGGGGG	50.0	47.4	58.2	54.0	1161-1181 bp	0.4	16.1
trnL-CAA-ndhF_p4	TCTCGCGTGTCTACCATTTC	TTCATGGTTTATCTGGGGG	50.0	47.4	57.7	54.0	1165-1185 bp	0.4	16.1
trnL-CAA-ndhF_p5	TCGCGTGTCTACCATTTCAC	TTCATGGTTTATCTGGGGG	50.0	47.4	58.3	54.0	1163-1183 bp	0.4	16.1