Hotspot

rps8-rpl14

LSC · 81935-82344 · 410 bp

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Pi 0.0823

MarkerSeek score 78.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 410
variable_sites: 58
pi: 0.0823
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTACAATCCCCATTCCGCC	GAACTCAAACGCGACAATGG	50.0	50.0	58.0	58.0	889-975 bp	1.0	89.0
rps8-rpl14_p2	ATTACAATCCCCATTCCGCC	GTTAAACCGGGCCGAATACT	50.0	50.0	58.0	58.0	1350-1436 bp	0.8	79.4
rps8-rpl14_p3	ACCGAACTCGAAACATACCG	GTTAAACCGGGCCGAATACT	50.0	50.0	58.0	58.0	1621-1707 bp	0.8	78.6
rps8-rpl14_p4	CCGAACTCGAAACATACCGT	GTTAAACCGGGCCGAATACT	50.0	50.0	58.0	58.0	1620-1706 bp	0.8	78.6
rps8-rpl14_p5	AAACTTCGCCGGATTCTACC	GTTAAACCGGGCCGAATACT	50.0	50.0	58.0	58.0	1676-1762 bp	0.8	78.3