Hotspot

psbB-psbT

LSC · 75151-75364 · 214 bp

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Pi 0.0516
MarkerSeek score 78.50
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
214
variable_sites
20
pi
0.0516
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 CGCCAGAACCCTGTTTAGAG GGAATGTATAAACCAATGCTTCCA 55.0 37.5 58.3 57.5 357-498 bp 1.0 47.1
psbB-psbT_p2 GCTCAAGTGGAATTTGGAGC GGAATGTATAAACCAATGCTTCCA 50.0 37.5 57.4 57.5 305-446 bp 1.0 45.7
psbB-psbT_p3 CGCCAGAACCCTGTTTAGAG AGGAATGTATAAACCAATGCTTCC 55.0 37.5 58.3 57.3 358-499 bp 1.0 45.1
psbB-psbT_p4 CGCCAGAACCCTGTTTAGAG GGAATGTATAAACCAATGCTTCCAT 55.0 36.0 58.3 57.9 357-498 bp 1.0 45.0
psbB-psbT_p5 CGCCAGAACCCTGTTTAGAG GGAATGTATAAACCAATGCTTCC 55.0 39.1 58.3 55.9 357-498 bp 1.0 45.0