Hotspot

rpl33-rps18

LSC · 68512-68780 · 269 bp

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Pi 0.067

MarkerSeek score 78.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 30
pi: 0.067
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACGATTCATGGGGAAATAAAGA	GGGGTAAACGCCTACGAAAA	36.4	50.0	55.5	58.2	331-388 bp	1.0	53.2
rpl33-rps18_p2	ACGATTCATGGGGAAATAAAGAA	GGGGTAAACGCCTACGAAAA	34.8	50.0	56.2	58.2	331-388 bp	1.0	51.1
rpl33-rps18_p3	ACGATTCATGGGGAAATAAAGA	CCGATCCAATTGGGGGTAAA	36.4	50.0	55.5	57.6	343-400 bp	1.0	51.0
rpl33-rps18_p4	ACGATTCATGGGGAAATAAAGA	ATCCAATTGGGGGTAAACGC	36.4	50.0	55.5	58.5	340-397 bp	1.0	50.7
rpl33-rps18_p5	ACGATTCATGGGGAAATAAAGA	ATTCGATCCCCCGATCCAAT	36.4	50.0	55.5	58.6	353-410 bp	1.0	50.5