Hotspot

accD-psaI

LSC · 58970-59881 · 912 bp

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Pi 0.0407

MarkerSeek score 74.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 59
pi: 0.0407
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CAGCTACAGGGTTTCCTTCC	CCACTAAAGGAACAAAAATAGAGGG	55.0	40.0	57.9	57.8	809-999 bp	1.0	49.3
accD-psaI_p2	CAGCTACAGGGTTTCCTTCC	CCCACTAAAGGAACAAAAATAGAGG	55.0	40.0	57.9	57.8	810-1000 bp	1.0	49.3
accD-psaI_p3	CAGCTACAGGGTTTCCTTCC	AGGAACAAAAATAGAGGGAAAGT	55.0	34.8	57.9	55.7	802-992 bp	1.0	49.3
accD-psaI_p4	CAGCTACAGGGTTTCCTTCC	GGAACAAAAATAGAGGGAAAGTTCA	55.0	36.0	57.9	57.7	801-991 bp	1.0	49.3
accD-psaI_p5	CAGCTACAGGGTTTCCTTCC	AGGAACAAAAATAGAGGGAAAGTTC	55.0	36.0	57.9	57.5	802-992 bp	1.0	49.3