Hotspot

atpB-rbcL

LSC · 54108-55228 · 1121 bp

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Pi 0.042
MarkerSeek score 78.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
1121
variable_sites
80
pi
0.042
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AGTCGTGGTATTGCTTCTCA GGTGACATAAGTCCCTCCCT 45.0 55.0 56.6 58.1 1171-1506 bp 1.0 76.7
atpB-rbcL_p2 AAGTCGTGGTATTGCTTCTCA GGTGACATAAGTCCCTCCCT 42.9 55.0 57.3 58.1 1172-1507 bp 1.0 74.6
atpB-rbcL_p3 AGTCGTGGTATTGCTTCTCA TGGTGACATAAGTCCCTCCC 45.0 55.0 56.6 58.4 1172-1507 bp 1.0 74.3
atpB-rbcL_p4 AGTCGTGGTATTGCTTCTCAT GGTGACATAAGTCCCTCCCT 42.9 55.0 57.1 58.1 1171-1506 bp 1.0 72.9
atpB-rbcL_p5 AAGTCGTGGTATTGCTTCTCA TGGTGACATAAGTCCCTCCC 42.9 55.0 57.3 58.4 1173-1508 bp 1.0 72.2