Hotspot

rps16-trnQ-UUG

LSC · 6329-7311 · 983 bp

Back to Phragmipedium

Pi 0.0378

MarkerSeek score 75.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 72
pi: 0.0378
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCGAGCTCCACCATATGCTA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1213-1511 bp	1.0	87.5
rps16-trnQ-UUG_p2	TCGAGCTCCACCATATGCTA	CGAAAACTTACGGCAGCTTG	50.0	50.0	58.0	58.0	1838-2136 bp	1.0	86.1
rps16-trnQ-UUG_p3	ATCCACAGCCAATCATGTCC	CGAAAACTTACGGCAGCTTG	50.0	50.0	57.9	58.0	1704-2002 bp	1.0	86.1
rps16-trnQ-UUG_p4	AATGGTGGATGGCAGAATCC	CGAAAACTTACGGCAGCTTG	50.0	50.0	57.9	58.0	1720-2018 bp	1.0	85.9
rps16-trnQ-UUG_p5	TCGAGCTCCACCATATGCTA	ATCGAAAACTTACGGCAGCT	50.0	45.0	58.0	57.9	1840-2138 bp	1.0	85.8