Hotspot

rps19-rpl2

IRb · 88094-88163 · 70 bp

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Pi 0.0263

MarkerSeek score 61.50

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 70
variable_sites: 5
pi: 0.0263
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCTCGGGACCAGGTTACTAT	GGGCCCCAATTGGTAGAAAA	55.0	50.0	58.6	58.1	80-325 bp	1.0	80.6
rps19-rpl2_p2	GCTCGGGACCAGGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	58.6	58.1	80-233 bp	1.0	80.6
rps19-rpl2_p3	GCTCGGGACCAGGTTACTAT	TTGGGGTTATCCTGCACTTG	55.0	50.0	58.6	57.8	80-291 bp	1.0	79.5
rps19-rpl2_p4	GCTCGGGACCAGGTTACTAT	ATTTGATTCTTCGTCGCCGT	55.0	45.0	58.6	58.3	80-235 bp	1.0	79.1
rps19-rpl2_p5	GCTCGGGACCAGGTTACTAT	TTTGATTCTTCGTCGCCGTA	55.0	45.0	58.6	57.6	80-234 bp	1.0	78.5